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鸡癌蛋白诱导转录物3(OIT3)酶联免疫吸附测定试剂盒
价格
订货量(96T)
￥1800.00
≥1
店铺主推品 热销潜力款
联系人 李经理 销售员 
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䀍䀑䀔-䀔䀑䀋䀋䀋䀐䀌䀐
发货地 江苏省南京市
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商品参数
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商品介绍
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联系方式
是否进口 进口原料
品牌 Camilo
用途范围 本试剂盒只能用于科学研究，不得用于医学诊断
实验方法 双抗体一步夹心法
有效期 6个月
贮藏 2-8℃，避光防潮保存
重复性 板内、板间变异系数均小于15%
特异性 不与其它可溶性结构类似物交叉反应
准确性 标准品线性回归与预期浓度相关系数R值，大于等于0.9600
商品介绍
鸡癌蛋白诱导转录物3(OIT3)酶联免疫吸附测定试剂盒
检测原理
试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。往预先包被人白介素10(IL-10)捕获抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并彻底洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的人白介素10(IL-10)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），计算样品浓度。
样品收集、处理及保存方法
1. 血清：使用不含热原和内毒素的试管，操作过程中避免任何细胞刺激，收集血液后，3000转离心10分钟将血清和红细胞迅速小心地分离。
2. 血浆：EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。
3. 细胞上清液：3000转离心10分钟去除颗粒和聚合物。
4. 组织匀浆：将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。
5. 保存：如果样本收集后不及时检测，请按一次用量分装，冻存于-20℃，避免反复冻融，在室温下解冻并确保样品均匀地充分解冻。
鸡癌蛋白诱导转录物3(OIT3)酶联免疫吸附测定试剂盒
试剂盒组成
| 名称 | 96孔配置 | 48孔配置 | 备注 |
| 微孔酶标板 | 12孔×8条 | 12孔×4条 | 无 |
| 标准品 | 0.5mL | 0.5mL | 按说明书进行稀释 |
| 标准品稀释液 | 6mL | 3mL | 按说明书进行稀释 |
| 样本稀释液 | 6mL | 3mL | 按说明书进行稀释 |
| 检测抗体-HRP | 6mL | 3mL | 无 |
| 20×洗涤缓冲液 | 20mL | 20mL | 按说明书进行稀释 |
| 底物A | 6mL | 3mL | 无 |
| 底物B | 6mL | 3mL | 无 |
| 终止液 | 6mL | 3mL | 无 |
| 封板膜 | 2张 | 2张 | 无 |
| 说明书 | 1份 | 1份 | 无 |
| 自封袋 | 1个 | 1个 | 无 |
鸡癌蛋白诱导转录物3(OIT3)酶联免疫吸附测定试剂盒
南京卡米洛生物工程有限公司，被行业内评为优秀企业，江苏实验室联盟企业,,江苏省质量信用AAA级企业。主要从事各种细胞因子酶联免疫试剂盒及其相关产品的研发，生产，销售和技术上的服务、培训与咨询。公司产品涉及许多生命科学研究领域，如胚胎学、血液学、免疫学和神经科学等等，特别是肿瘤研究领域。我们所研发的产品是致力于为中国生命科学研究领域的科学家们提供研究所需要的高品质产品，从而加速生物制药和生命医学市场的发展，提高疾病的诊断和治疗水平，以造福于全人类。公司一直致力于各种细胞因子及其相关产品的研发，生产和销售。卡米洛的产品覆盖面广，种类丰富，质量稳定可靠，其生产的各种ELISA试剂盒、重组因子及抗体以其卓越的品质赢得了广大科研院所及临床诊断机构的青睐。卡米洛生物工程坚持不懈的努力，已经研发出一大批优质的试剂。以最优质的试剂提供给广大科研工作者，是卡米洛生物一贯的愿望，也是做事的准则！公司的宗旨是“以人为本，质量第一，顾客至上”......
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联系方式
公司名称 南京卡米洛生物工程有限公司
联系卖家 李经理 
（QQ：3137331824）
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地址 江苏省南京市
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